Tips on using a micropipette

How to get the most out of your micropipette

The micropipette, if used correctly, is a fabulous tool for taking small volumes with great accuracy.  For best technique, consider the following tips:

  • Make sure your pipette is in good condition, and gets regularly calibrated (refer to your owner's manual for suggested time intervals)
  • Choose the right pipette for the right job. E.g.  For the Sentia device, a 100-1000 µl (0.1 mL - 1 mL) micropipette is adequate for the volume range to be covered.  DO NOT use a pipette past its volume limits.
  • DO use the correct pipette tips for your device and avoid roughly jamming them onto the pipette tip cone.  Jamming tips on can break the shaft.
  • DO keep your pipette clean to avoid contamination issues.  Most pipettes can be cleaned externally with typical laboratory cleaning agents, soap or alcohol (refer to your owner's manual).
  • Learn your plunger stops and good technique (stop 1 is used for aspiration/uptake and stop 2 for dispensing). * 
  • Practice using your pipette, ideally on water, prior to a first-time testing event.

* Correct pipetting technique instructions covered here.