How do I prepare a wine sample for acetic acid analysis?

A step-by-step guide for analyzing acetic acid on the Sentia™

To test for acetic acid on the Sentia you will need to dilute your sample 1:3 (1 part wine: 3 parts water).  

Equipment and solutions required:

  • Sample (wine)
  • Distilled or deionized water (reagent grade)
  • 1x small, clean vial 
  • Micropipette
  • 2x clean micropipette tips

Dilution instructions:

1. Using a micropipette with clean tip, accurately pipette 300µl (your 3-parts) distilled water into a clean vial.

2. Dispose of the used tip and replace.

3. Accurately pipette 100 µl (your 1-part) wine sample to the same vial

(Note: some samples may require a pre-degas step if highly carbonated)

4. Dispose of pipette tip.

6. Cap vial and gently shake to mix 

7. You now have a diluted wine sample ready to use. 

8. Dispose of waste wine and buffer solutions down the sink with running water.

For further information on how to perform an acetic acid dilution, please read our Guide to sample preparation for Sentia analysis or watch Preparing Diluted Samples for Sentia Analysis